Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen

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• 作者：Marinela Contreras^a ; ^{marinelacr@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Juan A. Moreno-Cid^a ; ¹ ; ^{drmorenocid@probelte.es" class="auth_mail" title="E-mail the corresponding author} ; Ana Domingos^b ; ^{adomingos@ihmt.unl.pt" class="auth_mail" title="E-mail the corresponding author} ; Mario Canales^a ; ² ; ^{mario.canales@probiovet.com" class="auth_mail" title="E-mail the corresponding author} ; Iratxe Dí ; ez-Delgado^a ; ^{Iratxe.Diez@uclm.es" class="auth_mail" title="E-mail the corresponding author} ; José ; M. Pé ; rez de la Lastra^a ; ³ ; ^{jm.perezdelalastra@csic.es" class="auth_mail" title="E-mail the corresponding author} ; Emilio Sá ; nchez^a ; ^{emiliouax@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Octá ; vio Merino^c ; ^{mero840125@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Rigoberto Ló ; pez Zavala^c ; ^{riglopez@uat.edu.mx" class="auth_mail" title="E-mail the corresponding author} ; Nieves Aylló ; n^a ; ^{Nieves.Ayllon@uclm.es" class="auth_mail" title="E-mail the corresponding author} ; Mariana Boadella^a ; ⁴ ; ^{mariana.boadella@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Margarita Villar^a ; ^{margaritam.villar@uclm.es" class="auth_mail" title="E-mail the corresponding author} ; Christian Gortá ; zar^a ; ^{Christian.Gortazar@uclm.es" class="auth_mail" title="E-mail the corresponding author} ; José ; de la Fuente^a ; ^d ; ^{jose_delafuente@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chimera ; Endotoxin ; Immunology ; MSP1a ; Subolesin ; Tick ; Vaccine
• 刊名：Ticks and Tick-borne Diseases
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：6
• 期：6
• 页码：820-828
• 全文大小：1496 K

文摘

Ticks are vectors of diseases that affect humans and animals worldwide. Tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. Recently, the Rhipicephalus microplus Subolesin (SUB)-Anaplasma marginale MSP1a chimeric antigen was produced in Escherichia coli as membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. In this research, lipidomics and proteomics characterization of the E. coli membrane-bound SUB-MSP1a antigen showed the presence of components with potential adjuvant effect. Furthermore, vaccination with membrane-free SUB-MSP1a and bacterial membranes containing SUB-MSP1a showed that bacterial membranes enhance the immunogenicity of the SUB-MSP1a antigen in animal models. R. microplus female ticks were capillary-fed with sera from pigs orally immunized with membrane-free SUB, membrane bound SUB-MSP1a and saline control. Ticks ingested antibodies added to the blood meal and the effect of these antibodies on reduction of tick weight was shown for membrane bound SUB-MSP1a but not SUB when compared to control. Using the simple and cost-effective process developed for the purification of membrane-bound SUB-MSP1a, endotoxin levels were within limits accepted for recombinant vaccines. These results provide further support for the development of tick vaccines using E. coli membranes exposing chimeric antigens such as SUB-MSP1a.