The Corynebacterium pseudotuberculosis genome contains two formamidopyrimidine-DNA glycosylase enzymes, only one of which recognizes and excises 8-oxoguanine lesion
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- 作者：Larissa Souza Arantesa ; 1 ; larissarantes1@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Liliane Gonç ; alves Vila Novaa ; 1 ; lgvilanova@yhaoo.com.br" class="auth_mail" title="E-mail the corresponding author ; Bruno Carvalho Resendea ; Mainá ; Bitarb ; mbitarufmg@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Ivan Evangelista Vale Coelhoa ; ivanglista2009@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author ; Anderson Miyoshic ; miyoshi@icb.ufmg.br" class="auth_mail" title="E-mail the corresponding author ; Vasco Ariston Azevedoc ; vascoariston@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Luciana Lara dos Santosa ; llaramg@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Carlos Renato Machadob ; crmachad@icb.ufmg.br" class="auth_mail" title="E-mail the corresponding author ; Dé ; bora de Oliveira Lopesa ; debora@ufsj.edu.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：BER ; base excision repair ; AP ; apurinic/apyrimidinic ; MutM ; formamidopyrimidine-DNA glycosylase ; ROS ; reactive oxygen species ; H2TH ; helix&ndash ; two-turn&ndash ; helix ; PDB ; Protein Data Bank ; OD ; optical density ; IPTG ; isopropyl b-d-thiogalactopyranoside ; PAGE ; polyacrylamide gel electrophoresis ; SDS ; sodium dodecyl sulfate ; PVDF ; polyvinylidene difluoride ; NBT ; nitro-blue tetrazolium ; BCIP ; 5-bromo-4-chloro-3-indolyl phosphate ; BLAST ; Basic Local Alignment Search Tool ; CFU ; colony-forming unit
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：233-243
- 全文大小：2541 K
文摘
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CpMutM1 but not CpMutM2 has important residues involved in recognition and excision of 8-oxoguanine.
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Overexpression of CpMutM1 or CpMutM2 proteins has a toxic effect on E. coli BH20 (mutM −).
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Only the CpMutM1 protein proved capable of recognizing and excising 8-oxoguanine damage.
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In silico-generated structures show a high degree of conservation between CpMutM1 and CpMutM2.
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Docking revealed a correct positioning of 8-oxoguanine at the active site of CpMutM1 but not CpMutM2.