The effect of cellular isolation and cryopreservation on the expression of markers identifying subsets of regulatory T cells
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- 作者：Weiying Zhang wzhang32@jhu.edu" class="auth_mail" title="E-mail the corresponding author ; Tricia L. Nilles tnilles1@jhu.edu" class="auth_mail" title="E-mail the corresponding author ; Jacquett R. Johnson jjohns20@jhu.edu" class="auth_mail" title="E-mail the corresponding author ; Joseph B. Margolick ; jmargol1@jhu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：CD4+ regulatory T cells ; CD120b ; CD39 ; PBMC cryopreservation ; HIV
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：431
- 期：Complete
- 页码：31-37
- 全文大小：1092 K
文摘
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CD120b expression on Tregs decreased after isolation and cryopreservation of PBMCs.
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The decrease after cryopreservation occurred when recovery of viable cells was low.
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In contrast, these processes did not affect expression of CD39 on Tregs.
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Low CD120b expression on freshly thawed Tregs was reversible by in vitro culture.
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These findings were seen in both HIV-infected and -uninfected donors.