Polyguluronate sulfate (PGS) effectively inhibits HBV replication in HepG2.2.15 cells.
PGS inhibited the expression and secretion of HBsAg and HBeAg with low cytotoxicity.
PGS can bind and enter into HepG2.2.15 cells to interfere with HBV transcription.
PGS can enhance the production and secretion of interferon β in HepG2.2.15 cells.
PGS may upregulate NF-κB and Raf/MEK/ERK pathways to enhance the interferon system.