vector-based RNAi triggers are stronger stimulators of IFN response than siRNAs.
most of the effects induced by vector-based reagents result from the transfection of plasmid DNA.
TLR3 and IFNα2 as specific molecular markers of immune response stimulation by siRNA and shRNA/sh-miRs, respectively.
poly(I:C) may not serve as a universal positive control of immune response stimulation in RNAi experiments.
transfection of RNAi triggers influences miRNA levels and the isomiRs composition