High-efficiency transduction and specific expression of ChR2opt for optogenetic manipulation of primary cortical neurons mediated by recombinant adeno-associated viruses
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- 作者：Lei Jina ; lei.jin@fz-juelich.de" class="auth_mail" title="E-mail the corresponding author ; Wienke Langea ; wienke.lange@web.de" class="auth_mail" title="E-mail the corresponding author ; Annika Kempmannb ; Annika.meisenberg@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Vanessa Maybecka ; v.maybeck@fz-juelich.de" class="auth_mail" title="E-mail the corresponding author ; Anne Gü ; ntherb ; guenther_a@gmx.net" class="auth_mail" title="E-mail the corresponding author ; Nadine Gruteserb ; nadine.gruteser@t-online.de" class="auth_mail" title="E-mail the corresponding author ; Arnd Baumannb ; a.baumann@fz-juelich.de" class="auth_mail" title="E-mail the corresponding author ; Andreas Offenhä ; ussera ; a.offenhaeusser@fz-juelich.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Electrophysiology ; Neuronal networks ; Channelrhodopsin
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 September 2016
- 年：2016
- 卷：233
- 期：Complete
- 页码：171-180
- 全文大小：2464 K
文摘
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A screen of adeno-associated virus (rAAV) serotypes found rAAV6 preferable for efficient gene delivery into cortical neurons.
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Specificity of neuronal expression was improved by use of the human Synapsin promoter (hSyn).
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Compared to CMV-ChR2opt electrofected neurons, higher temporal and spatial control was achieved with rAAV6-hSyn-ChR2opt.