Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles

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• 作者：Emma Portnoy ; MS^a ; ^b ; ^{portnoye@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Boris Polyak ; PhD^c ; ^d ; ^{Boris.Polyak@DrexelMed.edu" class="auth_mail" title="E-mail the corresponding author} ; Dorrit Inbar ; PhD^e ; ^{dorrit.inbar@mail.huji.ac.il" class="auth_mail" title="E-mail the corresponding author} ; Gilad Kenan ; MD^a ; ^e ; ^{giladankori@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Ahmad Rai ; MD^c ; ^{Ahmad.Rai@drexelmed.edu" class="auth_mail" title="E-mail the corresponding author} ; Suzanne L. Wehrli ; PhD^f ; ^{WEHRLI@email.chop.edu" class="auth_mail" title="E-mail the corresponding author} ; Timothy P.L. Roberts ; PhD^f ; ^{ROBERTSTIM@email.chop.edu" class="auth_mail" title="E-mail the corresponding author} ; Ameer Bishara ; MS^a ; ^{ameer.bi@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Aniv Mann ; MS^a ; ^{aniv.mnn@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Miriam Shmuel ; MS^a ; ^{mirishmuel55@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Katya Rozovsky ; MD^g ; ^{katro70@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Gal Itzhak^a ; ^{galitzhak@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Tamir Ben-Hur ; MD ; PhD^e ; ^{tamir@hadassah.org.il" class="auth_mail" title="E-mail the corresponding author} ; Shlomo Magdassi ; PhD^b ; ^{magdassi@mail.huji.ac.il" class="auth_mail" title="E-mail the corresponding author} ; Dana Ekstein ; MD ; PhD^e ; ¹ ; ^{dekstein@hadassah.org.il" class="auth_mail" title="E-mail the corresponding author} ; Sara Eyal ; PhD^a ; ^h ; ¹ ; ^{sarae@ekmd.huji.ac.il" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Imaging ; Epilepsy ; Magnetic nanoparticles ; Magnetic Resonance Imaging ; Inflammation
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：12
• 期：5
• 页码：1335-1345
• 全文大小：1875 K

文摘

Correct localization of epileptic foci can improve surgical outcome in patients with drug-resistant seizures. Our aim was to demonstrate that systemically injected nanoparticles identify activated immune cells, which have been reported to accumulate in epileptogenic brain tissue. Fluorescent and magnetite-labeled nanoparticles were injected intravenously to rats with lithium-pilocarpine-induced chronic epilepsy. Cerebral uptake was studied ex vivo by confocal microscopy and MRI. Cellular uptake and biological effects were characterized in vitro in murine monocytes and microglia cell lines. Microscopy confirmed that the nanoparticles selectively accumulate within myeloid cells in the hippocampus, in association with inflammation. The nanoparticle signal was also detectable by MRI. The in vitro studies demonstrate rapid nanoparticle uptake and good cellular tolerability. We show that nanoparticles can target myeloid cells in epileptogenic brain tissue. This system can contribute to pre-surgical and intra-surgical localization of epileptic foci, and assist in detecting immune system involvement in epilepsy.