Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins
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- 作者：Shveta Bathlaa ; 1 ; Preeti Rawata ; 1 ; Rubina Baithalua ; Munna Lal Yadava ; Jasmine Narub ; Anurag Tiwaria ; Sudarshan Kumara ; Ashok K. Balharac ; Surender Singha ; Suman Chaudharya ; Rajesh Kumarc ; Masoud Lotfana ; Pradip Beharea ; Sushil K. Phuliac ; Tushar K. Mohantya ; Jai K. Kaushika ; Shivramaiah Nallapetae ; Inderjeet Singhc ; Srinivas K. Ambatipudid ; Ashok K. Mohantya ; ashokmohanty1@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bovine ; Urine ; Proteome ; Profiling ; Shotgun proteomics ; In-gel ; In-solution
- 刊名：Journal of Proteomics
- 出版年：2015
- 出版时间：8 September 2015
- 年：2015
- 卷：127
- 期：part_PA
- 页码：193-201
- 全文大小：1024 K
文摘
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Ammonium sulphate, proteo spin and daifiltration methods used for protein extraction
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Ammonium sulphate precipitation method yielded maximum proteins.
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Identified a total of 1564 proteins in urine of healthy cows for the first time
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Comparison with human urine revealed 315 overlapping and 1256 new proteins.
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Biological significance and biomarker potential of cow urinary proteins reported