Construction and expression of an anti-VEGFR2 Nanobody-Fc fusionbody in NS0 host cell
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- 作者：Maryam Qasemia ; b ; Mahdi Behdanic ; Mohammad Ali Shokrgozara ; Vahid Molla-Kazemihaa ; Homa Mohseni-Kuchesfahanib ; kouchesfehani@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Mahdi Habibi-Anbouhia ; habibi_m@pasteur.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：Angiogenesis ; Cancer ; Nanobody-Fc fusion protein (fusionbody) ; VEGFR2
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：19-25
- 全文大小：1833 K
文摘
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We engineered a nano-Fc antibody coding sequence against VEGFR2.
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The construct transfected to NS0 host cell line and stable clones were selected by Zeocin selection medium.
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The supernatant collected and purified by protein A chromatography.
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Western blot and ELISA test confirmed the fusionbody's presence on purified sample.
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Flowcytometry analysis demonstrated the fusionbody's function in in vitro steps.