In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm

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• 作者：Azeana Zahari¹ ; Abdulwali Ablat² ; Yasodha Sivasothy¹ ; Jamaludin Mohamad² ; Muhammad I. Choudhary³ ; Khalijah Awang¹ ; ^{khalijah@um.edu.my" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bisbenzylisoquinoline ; Laurotetanine ; Norstephasubine ; Antiplasmodial ; Antioxidant ; Oxidative stress
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：9
• 期：4
• 页码：328-332
• 全文大小：449 K

文摘

To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromethane extract of Alseodaphne corneri.

Methods

Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography and preparative thin layer chromatography. The antiplasmodial activity of the isolated compounds was evaluated using the histidine-rich protein II assay. The isolated alkaloids were also tested for their antioxidant activity using three different assays; DPPH, ferric reducing ability of plasma and metal chelating assays.

Results

Malaria infection caused the formation of free radicals which subsequently led to oxidative stress and apoptosis. The antioxidant properties of the alkaloids under investigation revealed that in addition to the antiplasmodial activity, the alkaloids could also prevent oxidative stress. (+)-laurotetanine and (+)-norstephasubine exhibited strong antiplasmodial activities with IC₅₀ values of 0.189 and 0.116 μM, respectively.

Conclusions

Interestingly, the two most potent compounds that exhibit antiplasmodial activity also exhibit good antioxidant activities. The crude dichloromethane extract and the isolated compounds exert substantial antiplasmodial and antioxidative activities which in turn suppress oxidative stress and cause less damage to the host.