Effect of sulfide, osmotic, and thermal stresses on taurine transporter mRNA levels in the gills of the hydrothermal vent-specific mussel Bathymodiolus septemdierum

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• 作者：Ikumi Nakamura-Kusakabe^a ; ^{ikumik@aori.u-tokyo.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Toshihiro Nagasaki^a ; Azusa Kinjo^a ; Mieko Sassa^a ; Tomoko Koito^b ; Kei Okamura^c ; Shosei Yamagami^d ; Toshiro Yamanaka^d ; Shinji Tsuchida^e ; Koji Inoue^a
• 关键词：Taurine transporter ; Deep sea ; Environmental adaptation ; Hypotaurine ; Marine invertebrate ; Osmoregulation
• 刊名：Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：74-79
• 全文大小：871 K

文摘

Hydrothermal vent environmental conditions are characterized by high sulfide concentrations, fluctuating osmolality, and irregular temperature elevations caused by vent effluents. These parameters represent potential stressors for organisms that inhabit the area around hydrothermal vents. Here, we aimed to obtain a better understanding of the adaptation mechanisms of marine species to hydrothermal vent environments. Specifically, we examined the effect of sulfide, osmolality, and thermal stress on the expression of taurine transporter (TAUT) mRNA in the gill of the deep-sea mussel Bathymodiolus septemdierum, which is a dominant species around hydrothermal vent sites. We analyzed TAUT mRNA levels by quantitative real-time polymerase chain reaction (PCR) in the gill of mussels exposed to sulfide (0.1 or 1 mg/L Na₂S·9H₂O), hyper- (115% seawater) and hypo- (97.5%, 95.5%, and 85% seawater) osmotic conditions, and thermal stresses (12 °C and 20 °C) for 24 and 48 h. The results showed that mussels exposed to relatively low levels of sulfide (0.1 mg/L) and moderate heat stress (12 °C) exhibited higher TAUT mRNA levels than the control. Although hyper- and hypo-osmotic stress did not significantly change TAUT mRNA levels, slight induction was observed in mussels exposed to low osmolality. Our results indicate that TAUT is involved in the coping mechanism of mussels to various hydrothermal vent stresses.