Combining metabolic engineering and process optimization to improve production and secretion of fatty acids
详细信息 查看全文
- 作者：Rodrigo Ledesma-Amaroa ; rodrigo.ledesma@grignon.inra.fr" class="auth_mail" title="E-mail the corresponding author ; Remi Dulermoa ; Xochitl Niehusb ; Jean-Marc Nicauda ; jean-marc.nicaud@grignon.inra.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：TAG ; Triacylglycerol ; THIOs ; Thioesterases ; TGLs ; Triacylglycerol lipases DCW ; Dry Cell Weight ; FFA ; Free fatty acids
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：38
- 期：Complete
- 页码：38-46
- 全文大小：1756 K
文摘
- class="listitem" id="list_li0005">
- class="label">•
Metabolic engineering strategies permit fatty acid (FA) secretion in Y. lipolytica.
- class="label">•
Mimicking bacterial fatty acid production in Yarrowia allows secretion of 85% of FA.
- class="label">•
Alkane layer stimulates secretion and facilitates extraction and recovery of FA.
- class="label">•
Lipid synthesis was uncoupled to biomass formation allowing 120% of lipid content.