Two-phase anaerobic co-digestion (TPAD) showed good performance in effluent quality.
Different organic loading rates and hydraulic retention times (HRT) were carried out.
The performance of TPAcD was dependent on the HRT of the thermophilic digester.
Advantages of mesophilic and thermophilic anaerobic co-digestion have been obtained.
The increase in microbial activity is proportional to the organic loading rate.