Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production
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- 作者：Judit Willenbachera ; judit.willenbacher@kit.edu" class="auth_mail" title="E-mail the corresponding author ; Teresa Mohra ; teresa.mohr@student.kit.edu" class="auth_mail" title="E-mail the corresponding author ; Marius Henkelb ; marius.henkel@uni-hohenheim.de" class="auth_mail" title="E-mail the corresponding author ; Susanne Gebhardc ; s.gebhard@bath.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Thorsten Mascherd ; thorsten.mascher@tu-dresden.de" class="auth_mail" title="E-mail the corresponding author ; Christoph Syldatka ; christoph.syldatk@kit.edu" class="auth_mail" title="E-mail the corresponding author ; Rudolf Hausmannb ; rudolf.hausmann@uni-hohenheim.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Surfactin ; srfA operon ; Promoter exchange ; Bacillus subtilis ; Quorum sensing ; Biosurfactant
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 April 2016
- 年：2016
- 卷：224
- 期：Complete
- 页码：14-17
- 全文大小：363 K
文摘
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The promoter in front of the srfA operon was exchanged in two B. subtilis strains.
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A general enhancement of Surfactin production was not observed.
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Promoter substitution led to increased Surfactin yields in a minor producer strain.
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Product yields were reduced in a naturally strong Surfactin producer strain.
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Surfactin synthesis is differently regulated in minor and strong producer strains.