Single stranded siRNA (ss-siRNA) can adsorb on hydrophilic and hydrophobic surfaces.
ss-siRNA can self-assemble on surfaces through non-electrostatic interactions.
The ss-siRNA conformation depends on the nature of the interactions with the surface.
ss-siRNA can be loaded into uncharged and negatively charged nanocarriers.
Quantification of ss-siRNA loading can be biased with unconventional nanocarriers.