An LC/MS/MS method for the simultaneous determination of individual sphingolipid species in B cells
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- 作者：Si Mia ; Yuan-Yuan Zhaoa ; Rebecca F. Dielschneiderb ; c ; Spencer B. Gibsonb ; c ; Jonathan M. Curtisa ; jcurtis1@ualberta.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cer ; ceramide ; CLL ; Chronic Lymphocytic Leukemia ; dHCer ; dihydroceramide ; ELSD ; evaporating light scattering detector ; ESI ; electrospray ionization ; FA ; fatty acid ; GlcCer ; glucosylceramides ; GSL ; glycosphingolipid ; HILIC ; hydrophilic interaction chromatography ; IS ; internal standard ; LC/MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; LOD ; limit of detection ; LOQ ; limit of quantification ; MRM ; multiple reaction monitoring ; m/z ; mass-to-charge ratio ; PL ; phospholipid ; QC ; quality con
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：1031
- 期：Complete
- 页码：50-60
- 全文大小：1792 K
文摘
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An LC-ESI–MS/MS method was developed to investigate the profile of sphingolipids.
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The extraction method was modified to ensure the high recovery of all sphingolipids.
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33 molecular species were simultaneously analyzed in a single chromatographic run.
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The distribution of sphingolipids were different in healthy and cancerous cells.
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The method is easily adaptable to other biological sample types.