Protein-RNA Dynamics in the Central Junction Control 30S Ribosome Assembly
详细信息 查看全文
- 作者：Kris Ann Baker1 ; &dagger ; ; Rajan Lamichhane2 ; 5 ; &dagger ; ; Tek Lamichhane1 ; 2 ; 6 ; David Rueda2 ; 3 ; 4 ; david.rueda@imperial.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Philip R. Cunningham1 ; philc@wayne.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：rRNA ; ribosomal RNA ; smFRET ; single-molecule Fö ; rster or fluorescence resonance energy transfer ; PCR ; polymerase chain reaction ; WT ; wild type ; WC ; Watson&ndash ; Crick ; GFP ; green fluorescent protein ; rprotein ; ribosomal protein
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：11 September 2016
- 年：2016
- 卷：428
- 期：18
- 页码：3615-3631
- 全文大小：1478 K
文摘
- class="listitem" id="list_l0005">
- class="label">•
Study identified sequence, structure, and dynamic requirements of central junction.
- class="label">•
Cross-junction interactions especially a Watson–Crick base pair of 654:754 are required.
- class="label">•
Loss of ribosome function is linked to decreased and improper 30S assembly.
- class="label">•
Central domain folding requires S15, Mg2 +, and an ability to pivot at junction.
- class="label">•
Comprehensive results of central junction study can aid new antibiotic development.