Comparative study of muscle proteins in relation to the development of yake in three tropical tuna species yellowfin (Thunnus albacares), big eye (Thunnus obesus) and skipjack (Katsuwonus pelamis)
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Normal and burnt tuna muscles (BT) were compared by SDS–PAGE and western blot.

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SDS–PAGE patterns were species-specific.

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SDS–PAGE showed differences due to BT between big eye (BET) and yellowfin (YFT).

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A 43 kDa band showed the potential to be a good indicator for BT in BET and YFT.

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The 43 kDa band was identified as creatine kinase by proteomic analysis.

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