Normal and burnt tuna muscles (BT) were compared by SDS–PAGE and western blot.
SDS–PAGE patterns were species-specific.
SDS–PAGE showed differences due to BT between big eye (BET) and yellowfin (YFT).
A 43 kDa band showed the potential to be a good indicator for BT in BET and YFT.
The 43 kDa band was identified as creatine kinase by proteomic analysis.