The total alkaloid fraction of bulbs of Fritillaria cirrhosa displays anti-inflammatory activity and attenuates acute lung injury

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• 作者：Dongdong Wang^a ; ^b ; ^{wddong1988@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Jie Yang^c ; Qingdan Du^a ; Houcong Li^a ; Shu Wang^a ; ^{huaxiyaoxue3000@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Alkaloid ; Fritillaria cirrhosa ; Anti-inflammatory activity ; Acute lung injury
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：193
• 期：Complete
• 页码：150-158
• 全文大小：2739 K

文摘

Bulb of Fritillaria cirrhosa D.Don (BFC) has been wildly used in China for a long time for folk medicine since its significant therapeutic effects on respiratory diseases, such as cough, expectoration, pneumonia and bronchial inflammation, which are related to respiratory inflammatory response. However, there is a lack of investigation on the in vivo anti-inflammatory properties of BFC.

Aim of the study

The aim of this study was to evaluate the in vivo anti-inflammatory activity of the purified total alkaloid fraction of BFC (TAF) by using different animal models of inflammation to provide scientific evidence for its traditional use.

Materials and methods

The total alkaloid fraction from BFC was prepared by using H-103 resin column. Anti-inflammatory effect of TAF was evaluated by models of acetic acid-induced capillary permeability accentuation, carrageenan-induced rat paw edema, cotton pellet-induced granuloma formation and LPS-induced acute lung injury (ALI). The level of cytokines (TNF, IL-6, IL-4 and IL-10) was measured by ELISA. Histopathological analyses were performed by using hematoxylin and eosin staining.

Results

TAF can inhibit acetic acid-induced capillary permeability accentuation, carrageenan-induced paw edema, cotton pellet-induced granuloma formation, suppress inflammatory cells recruitment and cytokine production in the bronchoalveolar lavage fluid from LPS-induced ALI mice, and attenuate pathological changes in the lung tissues of ALI mice.

Conclusion

This study provides scientific evidence for bulb of F. cirrhosa to treat respiratory inflammation.