Characterization of microsatellites and repetitive flanking sequences (ReFS) from the topmouth culter (Culter alburnus Basilewsky)
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- 作者：Shi-Li Liua ; b ; Zhi-Min Gua ; guzhimin2006@163.com" class="auth_mail" title="E-mail the corresponding author ; Jin-Liang Zhaob ; jlzhao@shou.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yong-Yi Jiaa ; Wen-Ping Jianga ; Jian-Lin Guoa ; Qian Lia
- 关键词：Culter alburnus ; Microsatellite ; Transposable element ; Genetic diversity
- 刊名：Biochemical Systematics and Ecology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：63
- 期：Complete
- 页码：127-135
- 全文大小：977 K
文摘
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We used (CAA)8 and (GAA)8 probes to develop microsatellites for Culter alburnus.
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We obtained 159 microsatellite containing sequences (MCSs).
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Scans of MCSs showed cryptic repeated elements on unique flanking regions.
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Much of the cryptic repetitive DNA was identified as transposable elements.
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Nine microsatellites were polymorphic and tested on 30 cultured C. alburnus.