Antifungal activity of lactobacilli and its relationship with 3-phenyllactic acid production

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• 作者：O. Cort茅s-Zavaleta ; A. L贸pez-Malo ; A. Hern谩ndez-Mendoza ; H.S. Garc铆a
• 关键词：Antifungal agents ; Lactic acid bacteria ; 3-Phenyllactic acid
• 刊名：International Journal of Food Microbiology
• 出版年：3 March, 2014
• 年：2014
• 卷：173
• 期：Complete
• 页码：30-35
• 全文大小：646 K

文摘

In this study, 13 lactic acid bacteria (LAB) strains (including 5 Lactobacillus casei, 2 Lactobacillus rhamnosus, 2 Lactobacillus fermentum, 1 Lactobacillus acidophilus, 1 Lactobacillus plantarum, 1 Lactobacillus sakei, and 1 Lactobacillus reuteri species) were assessed for both their antifungal activity against four food spoilage molds (Colletotrichum gloeosporioides, Botrytis cinerea, Penicillium expansum, and Aspergillus flavus) and their capability to produce the novel antimicrobial compound 3-phenyllactic acid (PLA). Results demonstrated that all molds were sensitive to varying degrees to the cell-free supernatants (CFS) from LAB fermentations (p < 0.05), with growth inhibitions ranging from 2.65% to 66.82%. The inhibition ability of CFS was not affected by a heating treatment (121 掳C, 20 min); however, it declined markedly when the pH of CFS was adjusted to 6.5. With the exception of L. plantarum NRRL B-4496 and L. acidophilus ATCC-4495, all other LAB strains produced PLA ranging from 0.021 to 0.275 mM. The high minimum inhibitory concentration for commercial PLA (3.01-36.10 mM) suggests that it cannot be considered the only compound related with the antifungal potential of studied LAB and that synergistic effects may exist among other metabolism products.