cAMP inhibits migration, ruffling and paxillin accumulation in focal adhesions of pancreatic ductal adenocarcinoma cells: Effects of PKA and EPAC

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• 作者：Alex Burdyga ; Alan Conant ; Lee Haynes ; Jin Zhang ; Kees Jalink ; Robert Sutton ; John Neoptolemos ; Eithne Costello ; Alexei Tepikin
• 关键词：6Bnz-cAMP ; N6-benzoyl-cAMP ; 8Br-cAMP ; 8 ; 8-bromoadenosine-cAMP ; 8pCPT ; 8 ; 8-(4-chlorophenylthio)-2&prime ; -O-methyl-cAMP ; AKAR4 ; A-kinase activity reporter four ; DMEM ; Dulbecco's modified Eagle's medium ; EPAC ; exchange protein activated by cAMP ; FBS ; foetal bovine serum ; Frsk ; forskolin ; H84 ; CFP(nd)-EPAC(dDEP/CD)-Venus(d) ; IBMX ; 3-isobutyl-1-methylxanthine ; PBS ; phosphate buffered solution ; PDAC ; pancreatic ductal adenocarcinoma ; PKA ; protein kinase A
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
• 出版年：December, 2013
• 年：2013
• 卷：1833
• 期：12
• 页码：2664-2672
• 全文大小：1187 K

文摘

We demonstrated that increasing intracellular cAMP concentrations result in the inhibition of migration of PANC-1 and other pancreatic ductal adenocarcinoma (PDAC) cell types. The rise of cAMP was accompanied by rapid and reversible cessation of ruffling, by inhibition of focal adhesion turnover and by prominent loss of paxillin from focal adhesions. All these phenomena develop rapidly suggesting that cAMP effectors have a direct influence on the cellular migratory apparatus. The role of two primary cAMP effectors, exchange protein activated by cAMP (EPAC) and protein kinase A (PKA), in cAMP-mediated inhibition of PDAC cell migration and migration-associated processes was investigated. Experiments with selective activators of EPAC and PKA demonstrated that the inhibitory effect of cAMP on migration, ruffling, focal adhesion dynamics and paxillin localisation is mediated by PKA, whilst EPAC potentiates migration.