Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1
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- 作者：Sabrina Jutza ; Judith Leitnera ; Klaus Schmettererb ; Iago Doel-Perezc ; Otto Majdicd ; Katharina Grabmeier-Pfistershammere ; Wolfgang Pastera ; Johannes B. Huppac ; Peter Steinbergera ; peter.steinberger@meduniwien.ac.at" class="auth_mail" title="E-mail the corresponding author
- 关键词：ANOVA ; analysis of variance ; APC ; allophycocyanin ; CsA ; Cyclosporin A ; CAR ; chimeric antigen receptor ; CFP ; cyan fluorescent protein ; gMFI ; geometric mean of fluorescence intensity ; MP ; minimal promoter ; NIP45 ; NFAT-interacting protein ; RE ; response element ; TCS ; T cell stimulator cells ; TPR ; triple parameter reporter
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：430
- 期：Complete
- 页码：10-20
- 全文大小：1777 K
文摘
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A triple parameter reporter T cell line was generated.
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NF-κB, NFAT and AP-1 activity induces CFP, eGFP and mCherry, respectively.
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All three reporters were activated upon triggering of the TCR–CD3 complex.
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Costimulation via CD2 and CD28 enhanced NF-κB, NFAT and AP-1 reporter activity.
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PD-1 and BTLA signals inhibited NF-κB, NFAT and AP-1.