Enzymatic and metabolic engineering for efficient production of syringin, sinapyl alcohol 4-O-glucoside, in Arabidopsis thaliana

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• 作者：Yang Chu ; Tackmin Kwon ; Jaesung Nam ; ^{jnam@dau.ac.kr" class="auth_mail}
• 关键词：OE ; overexpression ; HPLC ; high performance liquid chromatography ; PSPG ; Putative Secondary Plant Glycosyltransferase ; UGT ; UDP-glucose ; alcohol glucosyltransferase ; F5H ; ferulate 5-hydroxylase ; PAL ; phenylalanine ammonia-lyase ; C4H ; cinnamate 4-hydroxylase ; 4CL ; 4-(hydroxy) cinnamoyl CoA ligase ; HCT ; hydroxycinnamoyl CoA ; shikimate/quinate hydroxycinammoyltransferase ; CHS ; chalcone synthase ; C3H ; p-coumarate 3-hydroxylase ; CCoAOMT ; caffeoyl CoA 3-O-methyltransferase ; CCR ; cinnamoyl CoA reductase
• 刊名：Phytochemistry
• 出版年：June, 2014
• 年：2014
• 卷：102
• 期：Complete
• 页码：55-63
• 全文大小：1847 K

文摘

To promote efficient production of syringin, a plant-derived bioactive monolignol glucoside, synergistic effects of enzymatic and metabolic engineering were combined. Recombinant UGT72E3/E2 chimeras, generated by exchanging parts of the C-terminal domain including the Putative Secondary Plant Glycosyltransferase (PSPG) motif of UGT72E3 and UGT72E2, were expressed in leaves of transgenic Arabidopsis plants; syringin production was measured in vivo and by enzymatic assays in vitro. In both tests, UGT72E3/2 displayed substrate specificity for sinapyl alcohol like the parental enzyme UGT72E3, and the syringin production was significantly increased compared to UGT72E3. In particular, in the in vitro assay, which was performed in the presence of a high concentration of sinapyl alcohol, the production of syringin by UGT72E3/2 was 4-fold higher than by UGT72E3. Furthermore, to enhance metabolic flow through the phenylpropanoid pathway and maintain a high basal concentration of sinapyl alcohol in the leaves, UGT72E3/2 was combined with the sinapyl alcohol synthesis pathway gene F5H encoding ferulate 5-hydroxylase and the lignin biosynthesis transcriptional activator MYB58. The resulting UGT72E3/2+F5H+MYB58 OE plants, which simultaneously overexpress these three genes, accumulated a 56-fold higher level of syringin in their leaves than wild-type plants.