First description of group A rotavirus from fecal samples of ostriches (Struthio camelus)

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• 作者：L.C. Silva ; A.A. Sanches ; F. Gregori ; P.E. Br ; ?o ; A.A. Alfieri ; S.A. Headley ; J.A. Jerez
• 关键词：Rotavirus ; Ostrich (Struthio camelus) ; Interspecies transmission ; G type ; P type
• 刊名：Research in Veterinary Science
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：93
• 期：2
• 页码：1066-1069
• 全文大小：165 K

文摘

This study investigated the occurrence of rotavirus infections in ostriches (Struthio camelus) reared in Northern Paran¨¢, Brazil. Fecal (n = 66) and serum (n = 182) samples from nine farms located in four different cities were analyzed by silver stained-polyacrylamide gel electrophoresis (ss-PAGE), RT-PCR assay, virus isolation, and counterimmunoelectroosmophoresis (CIE). Rotavirus group A seropositivity occurred in 5.49 % (10/182) of serum samples of ostriches originated from two farms. Only 9.09 % (6/66) of fecal samples from ostriches with diarrhea maintained in one farm were positive by ss-PAGE, RT-PCR, and virus isolation. The G (VP7) and P (VP4) genotypes of rotavirus wild strains isolated in cell culture were determined by multiplex-nested PCR. The genotyping identified two rotavirus strains: G6P[1] and G10P[1]. In three rotavirus strains it was only possible to identify the P type; one strain being P[1] and two strains that presented the combination of P[1] + P[7]. These findings might represent the first characterization of rotavirus in ostriches, and the finding of porcine and bovine-like rotavirus genotypes in ostriches might suggest virus reassortment and possible interspecies transmission.