Two-way molecular ligation for efficient conversion of monomeric hepatitis B virus DNA constructs into tandem dimers
详细信息 查看全文
- 作者：Li Zonga ; 1 ; Yanli Qinb ; 1 ; Haodi Jiaa ; 1 ; Huailiang Zhoua ; Chaoyang Chena ; Ke Qiaoa ; Jiming Zhangb ; Yongxiang Wanga ; Jisu Lic ; Shuping Tonga ; c ; shupingtong@fudan.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：cccDNA ; covalently closed circular DNA ; HBeAg ; hepatitis B e antigen ; HBsAg ; hepatitis B surface antigen ; HBV ; hepatitis B virus ; HCC ; hepatocellular carcinoma ; pg RNA ; pregenomic RNA
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：233
- 期：Complete
- 页码：46-50
- 全文大小：1163 K
文摘
- •
HBV DNA replication can be initiated from cloned tandem dimers but not monomers.
- •
Novel methods for converting a monomer into a tandem dimer were developed.
- •
Both methods involve the ligation of two rather than three molecules.
- •
About half of the bacterial colonies thus generated contained tandem dimers.