The signaling module cAMP/Epac/Rap1/PLCε/IP3 mobilizes acrosomal calcium during sperm exocytosis
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- 作者：Ornella Lucchesi ; Marí ; a C. Ruete ; Matí ; as A. Bustos1 ; Marí ; a F. Quevedo ; Claudia N. Tomes ; ctomes@fcm.uncu.edu.ar" class="auth_mail" title="E-mail the corresponding author
- 关键词：2-APB ; 2-aminoethoxydiphenylborate ; AR ; acrosome reaction ; 6-Bnz-cAMP ; N6-benzoyladenosine-3&prime ; 5&prime ; -cyclic monophosphate ; cAMP ; cyclic adenosine monophosphate ; Epac ; guanine nucleotide exchange factor activated by cAMP ; GEF ; guanine nucleotide exchange factor ; GST ; glutathione-S-transferase ; HTF ; human tubal fluid ; IP3 ; 1 ; 4 ; 5-trisphosphate ; IPTG ; isopropyl-β-d-thio-galactoside ; NP-EGTA-AM ; O-nitrophenyl EGTA-acetoxymethyl ester ; PBS ; phosphate-buffered saline ; 8-pCPT-2&prime ; -O-Me-cAMP
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular Cell Research
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：1863
- 期：4
- 页码：544-561
- 全文大小：4109 K
文摘
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The TAT-cAMP sponge is a suitable tool to unveil cAMP-related signaling pathways.
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Progesterone utilizes a cAMP-driven cascade and three small GTPases to elicit exocytosis.
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cAMP/Epac/Rap1/PLCε/IP3 constitute a signaling module in sperm.
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This module governs the intravesicular calcium mobilization essential for exocytosis.