Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing
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- 作者：Melissa L. Kelley ; Žaklina Strezoska ; Kaizhang He ; Annaleen Vermeulen ; Anja van Brabant Smith ; anja.smith@ge.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：RNA synthesis ; Gene editing ; Synthetic crRNA ; Synthetic tracrRNA ; Dual RNA
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 September 2016
- 年：2016
- 卷：233
- 期：Complete
- 页码：74-83
- 全文大小：2184 K
文摘
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CRISPR-Cas9 with synthetic crRNA:tracrRNA is an efficient system for gene editing.
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Chemically synthesized guide RNAs allow for incorporation of chemical modifications.
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Synthetic guide RNAs enable efficient high throughput arrayed screening.
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DNA-free gene editing is achievable with synthetic guide RNAs and Cas9 mRNA/protein.