Biochemical and histological evaluations of anti-inflammatory and antioxidant p-chloro-selenosteroid actions in acute murine models of inflammation

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• 作者：Marcel Henrique Marcondes Sari^a ; Ana Cristina Guerra Souza^c ; Suzan Gonç ; alves Rosa^a ; Pietro Maria Chagas^a ; Sô ; nia Cristina Almeida da Luz^b ; Oscar Endrigo Dorneles Rodrigues^a ; Cristina Wayne Nogueira^a ; ^{criswn@ufsm.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Organoselenium ; Selenium ; Inflammation ; Croton oil ; Carrageenan ; Mice
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：15 June 2016
• 年：2016
• 卷：781
• 期：Complete
• 页码：25-35
• 全文大小：2546 K

文摘

This study investigated the potential p-chloro-selenosteroid (PCS) anti-inflammatory effect in different animal models of acute inflammation. In order to determine a time- and a dose-curve response of action, female adult Swiss mice (25–35 g) were divided in different groups and pretreated by the intragastric route (i.g.) with PCS (5–10 mg/kg) and after the specific times (5, 30 and 60 min) the ear inflammation was induced with croton oil (2.5%, 20 μl). The ear edema, myeloperoxidase (MPO) activity and histological analyses were performed. In a second experiment, the pleurisy model was used to determine the PCS protective effect (10 mg/kg, i.g., 30 min before induction) in the inflammatory and oxidative alterations induced by an intrapleural injection of a 1% carrageenan solution (0.1 ml) in exudate and lung samples. Dexamethasone (1 mg/kg, i.g.) was used as positive control for both models. Statistical analysis was performed through a One-Way ANOVA test followed by the Newman–Keuls' test. Pretreatment of 30 min with PCS, only at a dose of 10 mg/kg, decreased ear edema and the MPO activity as well as the histological alterations induced by croton oil. In the pleurisy model, PCS (10 mg/kg, i.g.; 30 min) reduced the leukocyte counts, histological alterations, MPO and adenosine deaminase activities, oxidative damage and the non-enzymatic antioxidant defense imbalance. PCS had a similar anti-inflammatory profile to dexamethasone; however, it showed a better antioxidant effect. PCS had anti-inflammatory and antioxidant actions in two well established inflammation models in mice.