Effect of easily metabolizable sugars in the production of xylanase by Aspergillus tamarii in solid-state fermentation
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- 作者：Daniela Farani de Souza ; Cristina Giatti Marques de Souza ; Rosane Marina Peralta
- 关键词：Aspergillus tamarii ; Catabolite repression ; Glucose effect ; Solid-state fermentation ; Xylanase
- 刊名：Process Biochemistry
- 出版年：2001
- 出版时间：March 2001
- 年：2001
- 卷：36
- 期：8-9
- 页码：835-838
- 全文大小：107 K
文摘
The effect of easily metabolizable sugars (glucose, xylose, fructose, maltose, cellobiose and lactose) on xylanase production by Aspergillus tamarii in solid-state fermentation (SSF) was studied using wheat bran, corn cob and sugar cane bagasse as substrate. The addition of different sugars at a concentration of 1 % to sugar cane bagasse or corn cob media caused severe catabolic repression. In contrast, wheat bran systems were resistant to catabolic repression even at high concentrations of glucose (10 % ). In wheat bran cultures, glucose uptake was very efficient and less than 5 % of initial glucose concentration was present in the culture medium after 4 days of cultivation. In corn cob and sugar cane bagasse cultures, glucose uptake was slower and more than 60 % of the initial glucose concentration was present in the culture medium after 4 days of cultivation. These results demonstrate the importance of the nature of the substrate used in solid state cultures to obtain a system resistant to catabolic repression.