Exploiting dynamic reaction cell inductively coupled plasma mass spectrometry (DRC-ICP-MS) for sequential determination of trace elements in blood using a dilute-and-shoot procedure

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• 作者：Bruno Lemos Batista ; Jairo Lisboa Rodrigues ; Juliana Andrade Nunes ; Vanessa Cristina de Oliveira Souza ; Fern ; o Barbosa Jr.
• 关键词：Trace elements ; Interferences ; Inductively coupled plasma mass spectrometry ; Direct analysis ; Clinical specimens ; Dynamic reaction cell
• 刊名：Analytica Chimica Acta
• 出版年：2009
• 出版时间：20 April 2009
• 年：2009
• 卷：639
• 期：1-2
• 页码：13-18
• 全文大小：259 K

文摘

Inductively coupled plasma mass spectrometry with quadrupole (q-ICP-MS) and dynamic reaction cell (DRC-ICP-MS) were evaluated for sequential determination of As, Cd, Co, Cr, Cu, Mn, Pb, Se, Tl, V and Zn in blood. The method requires as little as 100 μL of blood. Prior to analysis, samples (100 μL) were diluted 1:50 in a solution containing 0.01 % (v/v) Triton^® X-100 and 0.5 % (v/v) nitric acid. The use of the DRC was only mandatory for Cr, Cu, V and Zn. For the other elements the equipment may be operated in a standard mode (q-ICP-MS). Ammonia was used as reaction gas. Selection of best flow rate of ammonium gas and optimization of the quadrupole dynamic band-pass tuning parameter (RPq) were carried out, using a ovine base blood for Cr and V and a synthetic matrix solution (SMS) for Zn and Cu diluted 1:50 and spiked to contain 1 μg L⁻¹ of each element. Method detection limits (3 s) for ⁷⁵As, ¹¹⁴Cd, ⁵⁹Co, ⁵¹Cr, ⁶³Cu ⁵⁵Mn, ²⁰⁸Pb, ⁸²Se, ²⁰⁵Tl, ⁵¹V, and ⁶⁴Zn were 14.0, 3.0, 11.0, 7.0, 280, 9.0, 3.0, 264, 0.7, 6.0 and 800 ng L⁻¹, respectively. Method validation was accomplished by the analysis of blood Reference Materials produced by the L’Institut National de Santé Publique du Quebec (Canada).