A two-stage label-free aptasensing platform was established to detect C. sakazakii.
This is the first report of an aptamer against C. sakazakii.
This assay can detect C. sakazakii in PIF at a 7.1 × 103 CFU/mL detection limit.
This assay requires only 30 min or less to complete after sample enrichment.
This platform may be applicable for detecting other bacteria in real-world samples.