Novel PCR-RFLP system based on rpoB gene for differentiation of Cronobacter species

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• 作者：Jiří ; Vlach ; Barbora Javůrková ; Ludmila Karamonová ; Martina Blažková ; ; ^{martina.blazkova@vscht.cz} ; Ladislav Fukal
• 关键词：Cronobacter spp. ; rpoB gene ; PCR-RFLP ; Species identification
• 刊名：Food Microbiology
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：62
• 期：Complete
• 页码：1-8
• 全文大小：643 K
• 卷排序：62

文摘

Bacteria from the genus Cronobacter are opportunistic foodborne pathogens. New primers for PCR-amplification of the rpoB gene were designed. Three endonucleases were applied for species-specific RFLP patterns analysis. A simple, cost-effective method is suitable for use in labs with standard equipment.