Engineering Escherichia coli for the synthesis of short- and medium-chain α,β-unsaturated carboxylic acids
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- 作者：Seohyoung Kima ; Seokjung Cheonga ; Ramon Gonzaleza ; b ; Ramon.Gonzalez@rice.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Metabolic engineering ; Synthetic biology ; β-oxidation reversal ; α ; β-unsaturated carboxylic acids ; 2-butenoic acid ; 2-hexenoic acid ; 2-octenoic acid ; 2-decenoic
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：36
- 期：Complete
- 页码：90-98
- 全文大小：1321 K
文摘
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A biological method for producing crotonic acid through reversal β-oxidation (r-BOX) was proposed.
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YdiI, an Escherichia coli thioesterase, was shown to convert trans-enoyl-CoAs to α,β-unsaturated carboxylic acids (α,β-UCAs).
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Production of medium chain α,β-UCAs through r-BOX platform was demonstrated.
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Superiority of r-BOX over fatty acid biosynthesis pathway was validated through the use of genome scale model.