We exposed D. magna to purified Cry1Ab and Cry2Aa toxins over the full life-span.
We used single toxins in different doses and combinations of toxins.
Animals exposed to Cry1Ab, Cry2Aa toxins at low ppm levels showed higher mortality.
Cry-toxins may have alternative modes-of-action in non-target organisms.
Cry-toxins acted in combination, stacked events may have stronger non-target effects.