Enhancement of solubility and yield of a β-glucan receptor Dectin-1 C-type lectin-like domain in Escherichia coli with a solubility-enhancement tag
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- 作者：Hari Prasad Dulala ; b ; Masamichi Nagaea ; Akemi Ikedaa ; Kana Morita-Matsumotoa ; Yoshiyuki Adachic ; Naohito Ohnoc ; Yoshiki Yamaguchia ; b ; yyoshiki@riken.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Dectin-1 ; GB1 tag ; Escherichia coli ; NMR ; Solubility enhancement
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：97-104
- 全文大小：1072 K
文摘
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Low solubility of Dectin-1 C-type lectin-like domain (CTLD) hinders NMR analysis.
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GB1 tag fusion to mDectin-1 CTLD enhances solubility 14-fold and expression 5-fold.
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NT-GB1 mDectin-1 CTLD retains β-glucan binding capacity without an effect of the tag.