Pulse exposure is environmentally relevant for aquatic toxicity testing of NPs.
Pulse exposure reduces Ag- and CuONP transformations during D. magna toxicity tests.
D. magna immobility 48 h after 3 h pulse was comparable to that after 24 h exposure.
AgNPs and AgNO3 pulses did not adversely affect reproduction in 21d post-exposure period.
CuONPs and CuCl2 pulses adversely affected reproduction in 21d post-exposure period.