Hydrothermal synthesis of copper based nanoparticles: Antimicrobial screening and interaction with DNA

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• 作者：K. Giannousi^a ; K. Lafazanis^b ; J. Arvanitidis^c ; A. Pantazaki^b ; ^{natasa@chem.auth.gr" class="auth_mail} ; C. Dendrinou-Samara^a ; ^{samkat@chem.auth.gr" class="auth_mail}
• 关键词：Copper based nanoparticles ; Antibacterial activity ; DNA degradation ; Reactive oxygen species ; Lipid peroxidation ; Ionic release
• 刊名：Journal of Inorganic Biochemistry
• 出版年：April, 2014
• 年：2014
• 卷：133
• 期：Complete
• 页码：24-32
• 全文大小：1085 K

文摘

Copper based nanoparticles (Cu-based NPs) of different compositions and sizes have been hydrothermally synthesized by varying the reaction time in the presence of the biocompatible surfactants polyoxyethylene (20) sorbitan laurate (Tween 20) and polyethylene glycol 8000 (PEG 8000). Effective control of the above synthetic parameters gave rise to Cu, Cu₂O and Cu/Cu₂O NPs of 10-44 nm. The antibacterial activity of the NPs was screened against Gram-positive (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus) and Gram-negative (Xanthomonas campestris, Escherichia coli) bacteria. The Cu-based NPs induce pDNA degradation in a dose-dependent manner as well as extensive ds CT-DNA degradation. Cu₂O NPs of 16 nm and 12 nm exhibit the lowest IC₅₀ values (2.13 渭g/mL and 3.7 渭g/mL) against B. cereus and B. subtilis, respectively. The agarose gel electrophoresis of ds CT-DNA treated with Cu₂O NPs demonstrated degradation at high concentration. In lower concentrations, viscosity measurements indicated groove binding. In regard to the enhanced antibacterial effect and specificity of Cu₂O NPs against the Gram-positive strains, the activity pathway was further explored and ROS production and lipid peroxidation verified. The released copper ions 5.15 mg/L in distilled water and 16.32 mg/L in nutrient medium, found below the critical value to inhibit bacterial growth and thus nanosized composition effect is predominant.