Distribution and molecular diversity of three cucurbit-infecting poleroviruses in China

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• 作者：Qiao-xia Shang ; Hai-ying Xiang ; Cheng-gui Han ; Da-wei Li ; Jia-lin Yu
• 关键词：Cucurbit polerovirus ; Identification ; Distribution ; Phylogenetic analyses ; Molecular diversity
• 刊名：Virus Research
• 出版年：2009
• 出版时间：November 2009
• 年：2009
• 卷：145
• 期：2
• 页码：341-346
• 全文大小：418 K

文摘

Cucurbit aphid-borne yellows virus (CABYV) and Melon aphid-borne yellows virus (MABYV) have been found to be associated with cucurbit yellowing disease in China. Our report identifies for the first time a third distinct polerovirus, tentatively named Suakwa aphid-borne yellows virus (SABYV), infecting Suakwa vegetable sponge. To better understand the distribution and molecular diversity of these three poleroviruses infecting cucurbits, a total of 214 cucurbitaceous crop samples were collected from 25 provinces in China, and were investigated by RT-PCR and sequencing. Of these, 108 samples tested positive for CABYV, while 40 samples from five provinces were positive for MABYV, and SABYV was detected in only 4 samples which were collected in the southern part of China. Forty-one PCR-amplified fragments containing a portion of the RdRp gene, intergenic NCR and CP gene were cloned and sequenced. Sequence comparisons showed that CABYV isolates shared 78.0–79.2 % nucleotide sequence identity with MABYV isolates, and 69.7–70.8 % with SABYV. Sequence identity between MABYV and SABYV was 73.3–76.5 % . In contrast, the nucleotide identities within each species were 93.2–98.7 % (CABYV), 98.1–99.9 % (MABYV), and 96.1–98.6 % (SABYV). Phylogenetic analyses revealed that the polerovirus isolates fit into three distinct groups, corresponding to the three species. The CABYV group could be further divided into two subgroups: the Asia subgroup and the Mediterranean subgroup, based on CP gene and partial RdRp gene sequences. Recombination analysis suggested that MABYV may be a recombinant virus.