- 作者：Diego Robles Mazzotti ; Cristiane Carvalho Singulane ; Vanessa Kiyomi Ota ; Thiago Potrich Rodrigues ; Tatiane Katsue Furuya ; Fern ; o Jos茅 de Souza ; Bruna Grassiela Cordeiro ; Camilla Magalh茫es de Oliveira Amaral ; Elizabeth Suchi Chen ; Anielli Jacomini ; Marilia de Arruda Cardoso Smith ; Bianca Borsatto-Galera
- 关键词：AD ; Alzheimer's disease ; APOE ; apolipoprotein E ; BMI ; body mass index ; CI ; confidence interval ; CVD ; cardiovascular disease ; GCPII ; glutamate carboxipeptidase II ; HWE ; Hardy Weinberg Equilibrium ; MMP9 ; matrix metalloproteinase ; OR ; odds ratio ; RFLP-PCR ; Restriction Fragment Length Polymorphism-Polymerase Chain Reaction ; SNP ; Single nucleotide polymorphism ; SSP-PCR ; Sequence-Specific Primer Polymerase Chain Reaction
- 刊名：Gene
- 出版年：10 February, 2014
- 年：2014
- 卷：535
- 期：2
- 页码：370-375
- 全文大小：259 K
Background and aims
The characterization of candidate gene polymorphisms in elderly populations is an important tool for the identification of risk factors for age-related diseases and conditions. We aimed to genotype the APOE polymorphisms (rs429358 and rs7412), rs61886492 (1561C>T) and rs202720 of GCPII gene and rs3918242 (鈭?#xA0;1562C>T) of MMP9 gene in an older-adult/elderly cohort from Cuiab谩 city, Mato Grosso Brazil as well as to characterize risk factors for morbidities and conditions affecting this cohort.Methods
The studied population consisted of 570 subjects from Cuiab谩 city, Brazil, who were subjected to clinical interviews and blood collection for laboratory examinations and DNA extraction. Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP-PCR), sequence-specific primer PCR (SSP-PCR) and TaqMan庐 allelic discrimination assay were used for genotyping.
Results
The frequencies of APOE 蔚2 and 蔚4 were 6.6% and 14.8%, respectively, and the frequencies of GCPII rs61886492 T allele, GCPII rs202720 C allele and MMP9 rs3918242 T allele were, respectively, 3.0%, 26.6% and 10.1%. Significant associations between APOE 蔚2 allele with lower total cholesterol and LDL-cholesterol were found. In addition, MMP9 rs3918242 T allele was associated with higher LDL-cholesterol levels, suggesting a link between lipid metabolism alteration and cardiovascular disease.
Conclusions
The present findings contributed to characterize risk factors specific for the studied population and to better understand the molecular physiopathology of common morbidities and conditions affecting older-adult/elderly people.