Experimental investigations on fluorescence excitation and depletion of ATTO 390 dye
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- 作者：Ding Luo ; Cuifang Kuang ; Xu Liu ; Guiren Wang
- 关键词：Fluorescence depletion efficiency ; Fluorescent dye ; Fluorescence microscope
- 刊名：Optics and Laser Technology
- 出版年：2013
- 出版时间：February, 2013
- 年：2013
- 卷：45
- 期：Complete
- 页码：723-725
- 全文大小：372 K
文摘
A novel fluorescent dye named ATTO 390 is tested and verified to be applicable in STED microscopy with great performance. The experimental configuration uses a 405 nm continuous wave (CW) laser as excitation beam and a 532 nm CW laser as depletion beam. The two collinear beams are focused on the dye using an objective with NA=1.4 and the fluorescence is detected by avalanche photodiode detector. With short wavelength in violet band, high fluorescence quantum yield, good photostability, and relatively low Ps demonstrated in a series of experiments, ATTO 390 is proved to be a potential good choice for fluorescent dye in STED microscopy.