Enhancing the resolution and contrast in CW-STED microscopy

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• 作者：Yue Fang ; Yifan Wang ; Cuifang Kuang ; ^{cfkuang@zju.edu.cn" class="auth_mail} ; Xu Liu
• 关键词：Subtraction method ; CW-STED microscopy ; Resolution ; Contrast
• 刊名：Optics Communications
• 出版年：1 July, 2014
• 年：2014
• 卷：322
• 期：Complete
• 页码：169-174
• 全文大小：7408 K

文摘

A new method based on the subtraction of images is proposed to enhance the resolution and contrast in CW-STED microscopy. It is realized by the subtraction of a confocal image from a STED image. Detailed theoretical analyses and a series of simulation tests are performed. The validity of this subtraction method in practice is demonstrated by experiments on both fluorescent nanoparticles and microtubule networks in which a better resolution and contrast than the STED image is achieved. Subtractive parameters are discussed in detail and a way of choosing the optimal value is proposed. Compared with other resolution enhancement concepts, this method is much simpler and free from using high intensity lasers.