Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection
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- 作者：Lei Tana ; Yuqiang Zhanga ; Fang Liua ; Yanmei Yuana ; Yuan Zhana ; Yingjie Suna ; Xusheng Qiua ; Chunchun Menga ; Cuiping Songa ; Chan Dinga ; b ; shoveldeen@shvri.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：IBV ; infectious bronchitis virus ; CMI ; cell-mediated immunity ; CTL ; cytotoxic T lymphocyte ; MHC ; major histocompatibility complex ; SPF ; specific-pathogen free ; ELD50 ; chicken embryo lethal dose ; EID50 ; median embryo infectious dose ; ATCC ; American Type Culture Collection ; DMEM ; Dulbecco&rsquo ; s Modified Eagle Medium ; RPMI1640 medium ; Roswell Park Memorial Institute ; FBS ; fetal bovine serum ; PBS ; phosphate-buffered saline ; mAb ; monoclonal antibodies ; CFSE ; carboxyfluorescein succinimidyl ; VN ; vir
- 刊名：Vaccine
- 出版年：2016
- 出版时间：17 October 2016
- 年：2016
- 卷：34
- 期：44
- 页码：5209-5216
- 全文大小：1047 K
文摘
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IBV S1 poly-epitope-based DNA incorporated neutralizing epitopes and BF2 restricted CTL epitopes were designed.
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This poly-epitope-vaccine could elicit the strong humoral and cellular immune responses against IBV stimulation in vitro.
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The poly-epitope-based DNA vaccine completely protects SPF chickens against lethal dose IBV challenge with SH1208 strain.