- 作者：Huiying Zhang ; Minli Lv ; Jiantao Jia ; Zhongfu Zhao ; Lili Zhang ; Lina Lai ; Yanjun Wu ; Baohong Li ; Chen Li ; Jingquan Ji ; Xiaoxia Tian ; Yan Liu ; Xujiong Li ; Hui Pang ; Jianhong Guo ; Limin Wang ; Yimin Fan ; Cuiying Zhang ; Dewu Han ; Cheng Ji
- 关键词：HPS ; hepatopulmonary syndrome ; ER ; endoplasmic reticulum ; IETM ; intestinal endotoxemia ; GRP78 ; 78 ; kD glucose-regulated protein ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; ECL ; enhanced chemiluminescence ; ALT ; alanine transferase ; MDA ; malondialdehyde ; TNF-伪 ; tumor necrosis factor-伪 ; CCl4 ; carbon tetrachloride ; ROS ; reactive oxygen species
- 刊名：Gene
- 出版年：1 March, 2014
- 年：2014
- 卷：537
- 期：1
- 页码：115-119
- 全文大小：581 K
Objective
This study is to explore the role of 78 kD glucose-regulated protein (GRP78) in the development of hepatopulmonary syndrome (HPS) in rats.Methods
The rat model of liver cirrhosis and HPS were induced with multiple pathogenic factors. Hematoxylin and eosin (H & E) staining was performed to detect the pathological changes of the lung and liver tissues. The levels of alanine transferase (ALT), endotoxin, and tumor necrosis factor-伪 (TNF-伪) in plasma and TNF-伪 and malondialdehyde (MDA) in lung tissues were detected. RT-PCR and Western blotting were conducted to detect the mRNA and protein expression levels of GRP78 in lungs.
Results
The plasma endotoxin level was gradually increased as HPS developed, and the mRNA and protein expression levels of GRP78 in lungs were also increased as the disease progressed. The levels of ALT and TNF-伪 in plasma and the contents of TNF-伪 and MDA in lung tissues were gradually increased along with the disease progression, with a strong positive correlation. Compared with controls, the plasma TNF-伪 level and the mRNA and protein expression levels of GRP78 in lung tissues were significantly higher in rats with HPS. The levels of endotoxin and ALT in plasma and the level of MDA in lungs were significantly higher in rats with HPS than controls.
Conclusions
The increased GRP78 expression is indicative of endoplasmic reticulum stress response during HPS, which may play an important role in the disease pathogenesis.