Theranostic tumor targeted nanoparticles combining drug delivery with dual near infrared and ¹⁹F magnetic resonance imaging modalities

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• 作者：Hieu Vu-Quang ; PhD^a ; ^b ; Mads Sloth Vinding ; PhD^a ; ^c ; Thomas Nielsen ; PhD^a ; ^c ; Marcus Gö ; rge Ullisch ; PhD^a ; ^c ; Niels Chr. Nielsen ; PhD^a ; ^b ; ^c ; ^d ; Jø ; rgen Kjems ; PhD^a ; ^b ; ^c ; ^e ; ^{kjems@inano.au.dk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：19F MRI ; PLGA ; ICG ; PFOB ; Doxorubicin ; Folate
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：12
• 期：7
• 页码：1873-1884
• 全文大小：1809 K

文摘

Combining imaging and drug delivery of “theranostic” nanoparticles has enabled concurrent diagnosis and therapy of diseases. Here, we describe a novel theranostic system that combines two imaging tracers, perfluorooctyl bromide (PFOB) for ¹⁹F magnetic resonance imaging (MRI) and indocyanine green (ICG) for near infrared (NIR) imaging, with the chemotherapeutic agent doxorubicin (Dox) into poly (lactic-co-glycolic acid)- poly (ethylene-glycol)-folate (PLGA-PEG-folate) nanoparticles. Cell culture studies using flow cytometry, confocal laser scanning microscope imaging, and ¹⁹F MRI showed enhanced uptake of nanoparticles via folate receptors expressed on human nasopharyngeal epidermal carcinoma (KB) cells. In vivo, higher MRI and fluorescence signals were obtained from tumors with ¹⁹F MRI and NIR, respectively, using folate-receptor-targeted nanoparticles compared with non-targeted equivalents. An in vitro cytotoxicity assay showed that folate-targeted nanoparticles were able to kill cancer cells more efficiently than non-folate conjugated particles. Our results suggest a potential use of PLGA-PEG-folate PFOB/ICG/Dox nanoparticles as a targeted chemotherapy agent traceable by either ¹⁹F MRI or NIR imaging.