文摘
Cell response to ALA-PDT was cell-type dependent in cell monolayers and 3D collagen cultures. ALA-PDT effect evaluated by WST-1 assay did not correlate with modified colony assay in Het-1A cells. ALA-PDT killed Kyse 450, but not Kyse 70 or Het-1A cells in 3D collagen gels, if analyzed 24 h after PDT. ALA induced PpIX fluorescence was detected in 3D collagen gels of Kyse 450, not Kyse 70 or Het-1A cells.