Real-time PCR quantification of Dehalococcoides populations: Methods and applications
详细信息 查看全文
- 作者：Alison M. Cupples
- 关键词：Bioremediation ; Chlorinated solvents ; Dehalococcoides ; Quantitative PCR ; Real-time PCR ; Reverse transcriptase real-time PCR
- 刊名：Journal of Microbiological Methods
- 出版年：2008
- 出版时间：January 2008
- 年：2008
- 卷：72
- 期：1
- 页码：1-11
- 全文大小：257 K
文摘
Dehalococcoides species are responsible for the reductive dehalogenation of an impressive range of common, persistent environmental contaminants. These microorganisms are difficult to both obtain and grow in pure culture, and so are often studied while they exist in consortia using molecular techniques. In particular, a significant number of quantitative real-time PCR (qPCR) assays targeting Dehalococcoides spp. have been reported. Initial qPCR methods targeted the 16S rRNA gene, however, because strains with the same 16S rRNA gene sequence can have different dehalogenating abilities, reductive dehalogenase genes are now emerging as the most appropriate qPCR target. Quantitative PCR has been critical to our current understanding of Dehalococcoides populations; it has provided information on their growth characteristics, dehalogenating abilities and effective use in bioremediation efforts. Future qPCR research directions will likely involve method standardization, as well as continued research on the functional genes associated with Dehalococcoides populations.