Fast high-performance liquid chromatography method for quality control of soy extracts

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• 作者：Apers ; Sandra ; Naessens ; Tania ; Van Den Steen ; Katleen ; Cuyckens ; Filip ; Claeys ; Magda ; Pieters ; Luc ; et. al.
• 关键词：Glycine max ; Food analysis ; Isoflavones ; Aglycones ; Glycosides
• 刊名：Journal of Chromatography A
• 出版年：2004
• 出版时间：June 4, 2004
• 年：2004
• 卷：1038
• 期：1-2
• 页码：107-112
• 全文大小：90.2 K

文摘

Soy extracts contain a mixture of isoflavones belonging to the group of phytoestrogens. In the quality control of soy the amount of isoflavones, both aglycones and glycosides, is usually determined by means of reversed-phase HPLC–UV. On conventional C₁₈-material columns, long analysis times are required in order to separate this complex mixture. In order to speed up analysis, the separation was optimized using two linked monolithic silica-based reversed-phase C₁₈ columns. A spectacular decrease of the analysis time, i.e. almost three-fold, was achieved by applying a flow rate of 3–4ml/min without loosing any separation efficiency. This analysis method for determination of isoflavones in soy extracts in less than 25min was fully validated according to the ICH guidelines.