Heterologous overexpression of a monotopic glucosyltransferase (MGS) induces fatty acid remodeling in Escherichia coli membranes
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- 作者：Candan Ariö ; za ; candan@dbb.su.se" class="auth_mail ; Hansjö ; rg Gö ; tzkea ; Ljubica Lindholma ; Jonny Erikssonb ; Katarina Edwardsb ; Daniel O. Daleya ; Andreas Bartha ; Å ; ke Wieslandera
- 关键词：MGS ; monoglucosyldiacylglycerol synthase ; PE ; phosphatidylethanolamine ; PG ; phosphatidylglycerol ; CL ; cardiolipin ; GlcDAG ; 伪-monoglucosyldiacylglycerol ; CFAs ; cyclopropanated fatty acids ; UFAs ; unsaturated fatty acids ; SFAs ; saturated fatty acids ; CFA ; cyclopropane fatty acid synthase ; FAME ; fatty acid methyl ester ; RpoE ; RNA polymerase subunit sigma 24 (蟽E) factor ; PlsB ; glycerol-3-phosphate acyltransferase ; PSD ; phosphatidylserine decarboxylase ; FabH ; 尾-ketoacyl-acyl carrier protein synthase
- 刊名：Biochimica et Biophysica Acta (BBA)/Biomembranes
- 出版年：July 2014
- 年：2014
- 卷：1838
- 期：7
- 页码：1862-1870
- 全文大小：1256 K
文摘
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Overexpression of MGS in Escherichia coli membranes changes fatty acid composition.
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Cyclopropanation is substantially decreased by MGS expression.
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cfa gene transcription is lowered in MGS expressing cells.
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BL21 AI and BL21 AI-MGS cells both maintain a liquid crystalline phase.