Viola plant cyclotide vigno 5 induces mitochondria-mediated apoptosis via cytochrome C release and caspases activation in cervical cancer cells

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• 作者：Mohammad Ali Esmaeili^a ; Nazanin Abagheri-Mahabadi^b ; Hossein Hashempour^c ; Mohsen Farhadpour^b ; Christian W. Gruber^d ; Alireza Ghassempour^b ; ^{a_ghassempour@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Apoptosis ; Cyclotide ; Mitochondrial pathway ; Viola ignobilis ; Vigno 5
• 刊名：Fitoterapia
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：109
• 期：Complete
• 页码：162-168
• 全文大小：946 K

文摘

Cyclotides describe a unique cyclic peptide family that displays a broad range of biological activities including uterotonic, anti-bacteria, anti-cancer and anti-HIV. The vigno cyclotides consist of vigno 1–10 were reported recently from Viola ignobilis. In the present study, we examined the effects of vigno 5, a natural cyclopeptide from V. ignobilis, on cervical cancer cells and the underlying mechanisms. We found that vigno 5-treated Hela cells were killed off by apoptosis in a dose-dependent manner within 24 h, and were characterized by the appearance of nuclear shrinkage, cleavage of poly (ADP-ribose) polymerase (PARP) and DNA fragmentation. The mitochondrial pathway of apoptosis revealed that cytochrome C is released from mitochondria to cytosol, associated with the activation of caspase-9 and -3, and the cleavage of poly (ADP-ribose) polymerase (PARP). Overall, the results indicate that vigno 5 induces apoptosis in part via the mitochondrial pathway, which is associated with a release of cytochrome C and elevated activity of caspase-9 and -3 in Hela cells.